longevity
Introducing Project Prima Donna: the first-ever treatment designed to regenerate lost enamel to keep you smiling for a lifetime. Enamel erosion is an irreversible, age-related condition that no one is immune from, leading to tooth sensitivity, cavities, and more severe health complications. Current solutions, such as fluoride rinses, crowns, or veneers, merely mask the problem without addressing the root cause.
Spearheaded by renowned stem cell researchers and dentists Dr. Hannele Ruohola-Baker, Dr. Julie Mathieu, and Dr. Jonathan An, this project utilizes breakthroughs in induced pluripotent stem cell (iPSC) technology to develop enamel-regenerating organoids. These organoids have shown promising results in the lab and are now ready for testing in a relevant animal model. This is an important step towards developing a drug that can move us beyond mere symptom management, to restore our natural ability to produce enamel.
The global economic burden of poor oral health was estimated to be $544 billion in 2015, and oral health is the most prevalent non-transmissible disease worldwide. If successful, this treatment stands to significantly reduce the global burden of dental diseases and healthcare costs. Join us on our journey to revolutionize oral health and to create a future where aging never dims your brilliant smile.
Enamel is like the prima donna of our body - brilliantly shining, the center of attention, but also quite temperamental. It's the toughest material in our body, acting as a shield for our teeth against damage, guarding the softer, sensitive layer underneath called dentin. Despite our best efforts with brushing and a healthy diet, enamel wears down as we get older. This wear can lead to sensitive and yellowing teeth, cavities, and even more severe systemic issues like heart disease and respiratory infections. Unfortunately, once enamel is gone, it's gone for good, as it cannot regrow. Studies show that poor oral health, including tooth discoloration, can significantly impact mental well-being and quality of life. Over 90% of people around the globe face enamel loss during their lives, with no hope of seeing their prima donna ever again.
Current methods of combatting enamel loss in the dental health industry vary based on how severe the erosion is:
While there are existing methods to repair enamel, they require continued application and fail to address the cause of enamel loss. Plus, the understudy can never beat the original when it comes to bite, comfort, and overall oral health. Thus, there is an unmet need for a method of treatment that regenerates lost enamel around teeth and prevents further enamel loss in aging humans, so they can keep smiling for a lifetime.
Project Prima Donna focuses on the widespread problem of enamel erosion. The team has already developed a novel co-culture protocol in the lab for growing cells into organoids that produce enamel. In addition, initial animal studies using mice have shown that these organoids produce enamel when placed inside the kidney capsule - a key first experiment to first see whether these cells would survive.
After these early successful steps, the team is now ready to test the organoids in a more relevant animal model, by placing these organoids within the teeth of animals who will have their enamel eroded. In this way, it will be determined whether it's possible to grow enamel in the tooth, in the exact location where it's needed. Upon successful completion of these studies, the team will use the data to identify key pathways that can be targeted by a drug, and will then work on developing novel chemistry to be used as a therapy, that can ideally be applied as a gel, foam, or rinse.
For now, our primary focus is on harnessing the power of stem cells to confirm whether they can indeed facilitate the formation and regeneration of tooth enamel, a concept that has remained largely theoretical until recently, due to the groundbreaking discoveries of our group.
This direct treatment contrasts with currently available methods that only protect the remaining enamel. There is no existing regenerative solution for enamel erosion, giving Project Prima Donna a unique advantage. This approach could improve dental health outcomes and cut long-term healthcare costs. The project will explore options for use both at home and by professionals, offering a significant upgrade from temporary fixes. The global market for dental consumables purchased by dentists for their offices was valued at USD 27.4 billion in 2021, with restorative and preventative materials and treatments, including professionally applied whiteners, fluoride rinses, implants, and veneers comprising more than 50% of the market revenue. With the increased longevity of the population and continued education on the necessity of proper oral hygiene, this market will only continue to grow.
The IP space surrounding enamel regeneration has little prior art and few competitors.
Current patents
In vitro models for enamel production
Patentable opportunities
Little competition in this space makes this a compelling, novel opportunity.
The team is in a unique position to match any funds that have been raised on Catalyst, up to $200,000. The total project plan is USD $474,204 and the team needs your help! They are prepared to match funds up to USD $200,000; $100,000 will be matched per milestone.
The project is broken down into 2 fundraising moments:
Therefore, only ~$200k is needed from supporters to fund ~$400k worth of work.
Item | Description | Timeline and cost |
---|---|---|
Milestone 1: Scale up production of organoids | The process for effectively scaling up production, storage, and characterization of the organoids is a key first step in ensuring sufficient amounts can be generated to a high quality for in vivo experiments. | 6 months $253,541 |
Milestone 2: Placement of organoids within teeth in vivo | 2 non-human primates will be used, as their teeth are most similar to humans. The animals will need 1 month to acclimate in the facility before experiments can start. Enamel will be removed at different depths within various teeth to assess different placement conditions. The teeth will be filled like a cavity to keep the organoids in place. The animals will be monitored to ensure no adverse reactions are observed prior to continuing into the monitoring phase. | 6 months combined with M3 |
Milestone 3: Validation of enamel production | Organoids will be left in place for at least 1 month to assess enamel growth. Regular X-rays will be taken as minimally invasively as possible as this can be combined with regular health check-ups for the animals. The teeth will eventually be extracted, and electron microscopy and histology will be performed to characterize and quantify enamel production. Tissues can be further processed for assessment of relevant signalling pathways in future experiments. | 4 months $220,664 |
Salaries and University overheads | Standard costs associated with sponsoring research at a university, including researcher salaries and administrative costs. | Included in milestone costs |
Liquidity Pool | 5% of the funding amount will go toward project liquidity - only for costs associated with Milestone 1 - $153,541 raise | $ 7,677 |
Total | 16 months $481,882 |
For the full funding application navigate to project documents.
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